Interpretations of the sample gels (pictures are in a separate file in the PCR module). Be sure to address: actual results (a description of what you see)
a statement that addresses whether or not the PCR was successful, unsuccessful, or partially successful, as well as an explanation as to why you think this. Use the DNA ladder picture provided in a separate document to determine whether the bands you see are the correct size.
if the PCR was unsuccessful or partially successful, one possible explanation as to why these results were obtained (other than water that was contaminated with bacterial DNA or nucleases)
a description of how you would troubleshoot the possibility detailed above – see BioRad for troubleshooting tips.
Answers
Answer:
A. Actual results: The first gel is a control gel, and the second gel is a sample gel. The control gel has a ladder of DNA bands of known sizes. The sample gel has bands of DNA that are of various sizes. The bands in the sample gel are not as distinct as the bands in the control gel.
B. A statement that addresses whether or not the PCR was successful, unsuccessful, or partially successful, as well as an explanation as to why you think this. The PCR was partially successful. The bands in the sample gel are of various sizes, which indicates that the PCR reaction was not able to amplify all of the DNA in the sample. This could be due to a number of factors, such as the DNA sample was not pure, The PCR reaction was not performed correctly, and The PCR primers were not specific to the target DNA.
C. Use the DNA ladder picture provided in a separate document to determine whether the bands you see are the correct size. The bands in the sample gel are of various sizes, but they are all within the range of the DNA ladder. This indicates that the PCR reaction was able to amplify DNA of various sizes.
D. If the PCR was unsuccessful or partially successful, one possible explanation as to why these results were obtained (other than water that was contaminated with bacterial DNA or nucleases). The PCR was partially successful. The bands in the sample gel are of various sizes, which indicates that the PCR reaction was not able to amplify all of the DNA in the sample. This could be due to a number of factors, such as the DNA sample was not pure, The PCR reaction was not performed correctly, and The PCR primers were not specific to the target DNA.
E. A description of how you would troubleshoot the possibility detailed above – see BioRad for troubleshooting tips. To troubleshoot the possibility that the DNA sample was not pure, I would repeat the PCR reaction with a new DNA sample that has been purified. To troubleshoot the possibility that the PCR reaction was not performed correctly, I would review the PCR protocol and make sure that all of the steps were followed correctly. To troubleshoot the possibility that the PCR primers were not specific to the target DNA, I would design new primers that are more specific to the target DNA.
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Related Questions
Which of the following would be considered the normal breathing rate for school-age children?
A.60 to 100 breaths per minute.
B.15 to 30 breaths per minute.
C.6 to 8 breaths per minute.
D.35 to 50 breaths per minute.
Answers
Answer:C
Explanation:
Which statement about natural resources is true Renewable resources need to be protected.
Renewable resources are difficult to replenish.
Conservation uses more natural resources than needed.
Nonrenewable resources are easily replaced.
Answers
What is the next name of the skull?
Answers
Answer:
Cranium
Explanation:
Answer:
cranium or braincase
Explanation:
what is cardio endurance
i only need like a little answer 7th grade
Answers
online use socratic by
Explanation:
spaceavant
Which of the following contributed most significantly to the ecological collapse described in the case of Easter Island?
Answers
Answer:
the drought, changing weather patterns, and human activities led to deforestation and subsequent soil erosion. These events precipitated the collapse of the Easter Island society.
Answer:
Humans arrived on the island.
Which long-term side effect is caused by using marijuana?
A. decreased risk of lung cancer
B. personality changes
C. decreased appetite
D. decreased heart rate
Answers
B) Personality alterations are a long-term side effect of marijuana use.
a long-term side effect caused by using marijuanaCannabis usage for a prolonged period of time has been linked to a number of long-term negative effects, including personality, mood, and behavior problems. According to research, those who use marijuana frequently for an extended period of time may go through personality changes, including a decline in motivation, an increase in apathy, and poor judgment.
Other long-term negative effects of marijuana use include breathing difficulties, diminished appetite, and slowed heartbeat. The long-term side effect of marijuana usage, however, is not a reduced chance of lung cancer. In truth, marijuana smoking can damage the lungs and raise the risk of lung cancer.
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Answer:
personality changes
Explanation: I did the test k12
Maisie is beginning to resent Gianna for her judgmental attitude and sarcasm. However, she continues to communicate and be friendly with her because as the most popular girl in school, Gianna could turn everyone against Maisie. This is an example of
A. dynamism
B. adapting
C. maladapting
D. deal breaking
Answers
Where do you go to find peace and quiet? Who has the ability to bring calm or peace of mind to you?
Answers
Answer:
(For this very first part, you need to give in your personal answer). People who are in a great social or overall a very connected person can really bring impact onto aspects like peace of mind. when such connected people like loved ones or friends take obligation over your mental emotions then the acquisition of peace of mind is found.
Explanation:
hope this helps!!
The high inflation rates of the late 1960s and early 1970s were due in part to
Answers
Answer:
the rising of oil prices.
Explanation:
the price of oil has tripled in pricing and were due to rising wages.
The high inflation rates of the late 1960s and early 1970s were due in part to the cause of increased rate of oil.
What is the inflation rates ?
India Inflation Rate Highest Since October 2020. Annual inflation charge in India elevated to 6.95% in March of 2022, the best seeing that October of 2020, and above marketplace forecasts of 6.35%
After the great oil shocks, oil prices quadrupled in 1970s and doubled in 1980s. The combination of high inflation and weak economic growth, fueled by repeated supply shocks, led to the phenomenon of 'stagflation'.
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The group responsible for the inspection and grading of meat is the.
Answers
Answer:
USDA - United States Department of Agriculture
The USDA's Agricultural Marketing Service is the agency responsible for grading meat and poultry.
What is grading of meat in meat industry?In simple term grading is the selection of animals for slaughter and dividing them into different grades depending on some criteria.
What does USDA inspected mean?The Federal Meat Inspection Act (FMIA) requires that all meat sold commercially be inspected and passed to ensure that it is safe, wholesome, and properly labeled.
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stress is considered a good stress when
Answers
it helps give someone the push that they need so they can pursue their goal/dream
Explanation:
I hope this helps if I am wrong I am very sorry!!
suggest three negative effects of destroying the water
Answers
Need help ASAP!! This is worth 40% of my grade!!!
Create a "healthy" meal that YOU would eat ensuring there is a food from each food group.
NOTE: In this assignment, do not choose entrees consisting of multiple food groups like a cheese burger, etc.
Answers
Answer:
Grain bowls.
Veggie loaded frittatas. dinner salad.
Loaded brown rice pasta.
One-pot soups.
Curry
Explanation:
These all have low calories and good nutrition.
Those wishing to receive a motorcycle license must
apply for a motorcycle learner's permit.
pass an approved motorcycle safety course.
verify 50 hours of motorcycle experience.
pass a physical examination.
Answers
Answer:
I think the answer would be the first one
Answer:
im pretty sure its all
Explanation:
Based on the textbook's discussion, ______ may help us better manage stress, whereas ______ may help us experience less stress in the first place.
Answers
Building social support may help us better manage stress, according to the discussion in the textbook, although aerobic activity may help us experience less stress overall.
What is meant by social support?Your family, friends, coworkers, and others provide you with social support in the form of physical and mental comfort.Social support is the belief and experience that one is supported, that help is available from others, and, most commonly, that one is part of a social network that is encouraging. These helpful resources can be material or intangible, emotional or informative, or companionship. Social support encompasses interpersonal interaction, respect and acceptance, love and understanding, concern and care, affection and company, and financial support.Make time for your loved ones and friends. Say hello or offer to help. People are more inclined to be there for you if you are there for them.To learn more about social support, refer to:
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When choosing between continuing an argument and walking away, which situation supports walking away as the better option?
A trusted friend is mediating.
There is pressure to act quickly.
The argument is likely to be resolved.
Both individuals are speaking respectfully.
Answers
Answer:
B.) There is pressure to act quickly
Explanation:
Option A is incorrect
there is no conflict.Option B is correct
if a situation is escalated to the point where one feels pressured to act quickly, leaving the situation altogether would be the best option, instead of continuing it.Option C is incorrect
there is communication and understanding between the parties that is leading towards a solution. There would be no reason to walk away.Option D is incorrect
if an altercation is handled in a respectable manner, walking away wouldn't be necessary to diffuse things.If both individuals are speaking respectfully, then it would be better to continue the argument and try to reach a resolution. Therefor, option (D) is correct.
Why having a conversation important?Walking away from a respectful conversation can make the other person feel unheard or disrespected, and it may prevent the two individuals from reaching a mutually beneficial agreement. Walking away may be a better option if there is pressure to act quickly and there is no time to discuss the issue thoroughly, or if the argument is likely to escalate into a more heated or aggressive confrontation.
In such situations, it may be more productive to take a break, cool down, and revisit the conversation at a later time when emotions are not running as high. If a trusted friend is mediating, it may be beneficial to continue the conversation with their guidance and support.
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Publish your educational campaign on the Internet, on social media, in a PowerPoint
presentation, within a Word document, or using another technological platform.
Proofread your campaign to look for grammar and punctuation errors. Make sure
that members of your group test your campaign to make sure everything is working
as it should. Share the campaign with a few people and ask them to review it. Ask
them to list any questions they have about using the campaign or the information.
That way, you can review your product with people who weren't involved in creating
it.
Once you have reviewed and made any necessary revisions to your campaign,
submit the campaign and this completed worksheet to your teacher for grading.
Answers
My educational campaign will be based on the topic: The education of the Girl Child.
How will the educational campaign be like?First of all, the educational campaign of the girl child will be publish on Internet, as well as some social media and it will be in a PowerPoint format.
I will make the presentation such that it will list the relevance of the education of the girl child across the work especially in Africa and Arab nations.
It will in a format that looks like a call to action and data will be obtained using questionnaires, research articles and other website documentation.
A girl's education entails molding her mind, character, and abilities. Every girl child should have access to education as a fundamental human right, regardless of age or nationality.
Therefore, When a woman is educated, her greater earning potential can increase her status within her family and community, allowing her to have a greater voice in crucial decisions. Additionally, it provides her more self-assurance to defend herself and act in her children's and her own best interests.
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A woman currently gets out of breath while causally walking one mile in 30 minutes she wants to improve her fitness level
Answers
When rick pops a handful of peanuts into his mouth at the ballgame, he is engaging specifically in the process of:_______.
Answers
When rick pops a handful of peanuts into his mouth at the ballgame, he is engaging specifically in the process of ingestion.
What is ingestion?Ingestion is the process of consuming any substance by an organism by swallowing or absorbing it.Mostly it is accomplished by eating or drinking (e.g. food, water, medicine, etc.) through the mouth into the GI tract.Single cell organisms ingest by absorbing the substances through their cell membrane.Ingestion is the first process of digestion.After ingestion, food is broken down physically (mastication) and chemically (by enzymes).The digested foods are then absorbed through the blood to the cells.The digested foods are then converted into the parts of a cell.The undigested products are then excreted out as feces.Learn more about digestion here:
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What do the additional needs add to the hierarchy? In your opinion are these additions needed? Explain?
Answers
Answer:
Additional needs are the secondary needs which are not needed for survival but are needed for the development and to provide ourself with happiness and comfort.The additional needs are love ,affection ,self actualization,freedom,self esteem etc .Besides ,basic needs it is must to have family,affection,belongingess,love,relationship,achievements,status,responsibility,knowledge,beauty,personal growth,self fulfillment and helping others to self actualise.These are the only qualities that helps us to be able to find meaning and purpose in our life and able to say that 'i trult lived'so ,yes in my opinion these additional needs are also needed along with basic needs
Explanation:
When this procedure is performed, one technique is to take multiple samples and calculate the average. Discuss why this method is important in blood typing.
Answers
Answer:
Taking multiple samples and calculating the average is an important method in blood typing because it helps to reduce the chance of errors and provides more accurate results.
Blood typing involves testing for the presence or absence of certain antigens on the surface of red blood cells. The presence or absence of these antigens determines a person's blood type. However, the amount of these antigens can vary from person to person, and sometimes they may be present in very low levels.
By taking multiple samples and calculating the average, it is more likely that the results will be accurate and reliable. This is particularly important in situations where blood transfusions or organ transplants are being considered, as an incorrect blood type match can be life-threatening.
In addition, taking multiple samples and calculating the average can also help to detect any errors or inconsistencies in the testing process. If there is a discrepancy between the results of different samples, it may indicate a problem with the testing procedure, which can then be corrected.
When Carbohydrates are processed by the body, the body turns them into this usable sugar for the body:
Answers
Answer:
The correct answer is - glucose.
Explanation:
When carbohydrate is processed or digested then it breaks down into its monomer and this monomer is mainly glucose as it is utilized by the body to produce energy in the process of cellular respiration and release ATP.
Glucose is the sugar that is stored in the body by breakdown and absorption of the uses to produce energy when the body requires it.
The correct answer is - glucose.
50 POINTS + BRAINLIEST!
________ protects our organs, insulates our bodies, and help carry vitamins A, D, E, and K.
Fat
Protein
Sugar
Water
Answers
Answer:
Fat is the correct answer.
Explanation:
Fat protects our organs, insulates our bodies, and help carry vitamins A, D, E, and K.
Fat fills your fat cells and insulates your body to help keep you warm.
Explanation:
fat protects our organs, insulates our bodies, and help carry vitamins A, D, E, and K
The tissue that forms the inner lining of the respiratory passages is
Answers
sand that can be used to make long Ribbons has
Answers
Answer:
The soil that can be used to make long ribbons has
A. Cracks when dry
B. Large air spaces
C. Rough texture
D. Low capillarity
What are some reasons Hill gives for being a vegetarian?
Answers
Answer:
The way animals are killed, what they are put through, and their own health.
Explanation:
Information literacy is very vital in this technological era due to Infobesity. A health professional is required to be information literate so that he/she can perform excellently at work. Discuss with relevant examples, four (4) reasons why Information literacy skills is invaluable to health professionals especially midwives.
Answers
Information literacy skills are invaluable to health professionals, especially midwives, as they enable them to navigate the vast amount of information, make evidence-based decisions, and provide quality care.
In today's technology-driven world, health professionals, including midwives, face a constant influx of information. Here are four reasons why information literacy skills are invaluable to health professionals, especially midwives:
1. Informed Decision Making: Information literacy skills empower midwives to critically evaluate and analyze a vast amount of information. They can assess the credibility, relevance, and quality of sources, allowing them to make evidence-based decisions in providing care to mothers and newborns.
2. Staying Updated: Information literacy skills enable midwives to keep abreast of the latest research, guidelines, and best practices in their field. By accessing reliable and up-to-date information, they can provide the highest standard of care, incorporate new techniques, and adapt to emerging healthcare trends.
3. Patient Safety: Midwives equipped with information literacy skills can identify and apply evidence-based interventions, protocols, and guidelines to ensure patient safety. They can access accurate information on medications, procedures, and potential risks, enabling them to make informed decisions that minimize adverse outcomes.
4. Effective Communication: Information literacy skills enhance midwives' ability to communicate health information effectively. They can access reliable sources to gather evidence and share accurate information with patients, families, and colleagues.
Hence, information literacy skills are invaluable to health professionals, especially midwives, as they enable them to make informed decisions, stay updated, ensure patient safety, and effectively communicate health information in the era of Infobesity.
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can I have some help ?
Answers
Answer:
what are you supposed to do?
A client has identified a goal for treatment that they estimate will take about a year to achieve. What is the best way to facilitate a plan of care that is dynamic, evolving, and pertinent to the clinical process?
Answers
A client has identified a goal for treatment that they estimate will take about a year to achieve. the best way to facilitate a plan of care that is Patient-centered care, dynamic, evolving, and pertinent to the clinical process are as follows:
What is Patient-centered care?Patient-centered care is described as "care that is respectful of and attentive to individual patient preferences, needs, and values" by the Institute of Medicine.
We slightly modified this definition to say that patient-centered care is defined as care that "honors and responds to individual patient preferences, needs, values, and goals." This was done after a series of focus groups with patients, iterative feedback from research colleagues, and consultation with national advisers. We explain why and how patient-centered care should be a requirement for all health care systems via this lens, regardless of whether that "system" is a solo practitioner, a sizable multispecialty group practice, or a federally certified health center that offers care to marginalized populations.To know more about Patient-centered care visit
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These figures are similar. The perimeter and
area of one are given. The perimeter of the
other is also given. Find its area and round
to the nearest tenth.
Perimeter = 20 cm
Area = 18.6 cm?
Perimeter = 28 cm
Area = [? ]cm
Answers
Answer:
36.5 cm²
Explanation:
The perimeter of the smaller shape = 20 cm and The area of the smaller shape = 18.6 cm²
The perimeter of the bigger shape = 28 cm
The length and breadth of the smaller shape is increased by a factor of k to produce the larger shape.
If the length of the smaller shape = l and its width = w. The perimeter of the smaller shape = 2(l + w) = 20
Since The length and breadth of the smaller shape is increased by a factor of k to produce the larger shape, the length of the larger shape = kl and its width = kw. Therefore the perimeter of the larger shape = 2(kl + kw) = 2k (l + w) = 28 cm
To find k, divide the perimeter of the larger shape by the perimeter of the larger shape:
\(\frac{2k(l+w)}{2(l+w)} =\frac{28}{20} \\k=1.4\)
The area of the smaller shape = l * w = 18.6 cm²
The area of the larger shape = kl * kw = k² * l * w = 1.4² * 18.6 cm² = 36.5 cm²