In order to bind to DEAE-cellulose at pH 7 , a protein must be: a. Neutral b. Negatively Charged c. Positively Charged d. None of the above
Answers
In order to bind to DEAE-cellulose at pH 7, a protein must be positively charged. The DEAE- cellulose beads are used to isolate and purify proteins with a positive charge. In biochemistry, DEAE-cellulose (Diethylaminoethyl cellulose) is a type of ion-exchange resin used for separating biomolecules based on differences in net surface charge.
The purification principle is based on the fact that DEAE-cellulose has a positive charge that can bind negatively charged protein molecules. The technique depends on the attraction between DEAE-cellulose and proteins, which are negatively charged at the physiological pH. They are adsorbed onto the DEAE-cellulose. A buffer of high salt concentration (i.e., NaCl) is used to elute the protein bound to DEAE-cellulose.
A buffer solution is a chemical solution containing a weak acid and its related base, or a weak base and its related acid. Buffer solutions are commonly used in laboratory settings to preserve a steady pH.
A buffer solution resists a change in pH by absorbing excess hydrogen or hydroxide ions. A buffer is a substance that maintains a constant pH when a small quantity of an acidic or basic material is introduced into it, thus preventing any change in pH.The answer to the question "In order to bind to DEAE-cellulose at pH 7, a protein must be" is c. Positively
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Related Questions
When considering different hypotheses, usually the
_______ one which can account for the
________ is the correct one.
Answers
Simplistic, information
Answer:
simplest, information
WHY do dolphins hate sharks
Answers
Why do sharks avoid Dolphins?
Made of very strong and thick bone, dolphin snouts are biological battering rams. Dolphins will position themselves several yards under a shark and burst upwards jabbing their snout into the soft underbelly of the shark causing serious internal injuries. More than Peas in a Pod.
both euglenas and volvox are classified as plant-like protists. but volvox has more structures in common with plants than euglenas do. which structure found in plant cells is also found in volvox but not in euglenas?
Answers
Both Euglenas and Volvox are classified as plant-like protista, and Volvox is more closely related to plants than is Euglena because Volvox is chlorophytic, potoautotrophic, found in freshwater, and a type of algae.
What are the plant's characteristics?The plant cell has chlorophyll that does the photosynthesizing, a vacuole, and other organelles. Many connecting links were discovered during the course of evolution, such as the volvox, which exhibits some plant-like characteristics, such as being an algae with photosynthetic capacity.
Hence, both Euglenas and Volvox are classified as plant-like protists, and Volvox is more closely related to plants than is Euglena because Volvox is chlorophytic, found in freshwater, potoautotrophic, and a type of algae.
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Why is heating a first step in pcr amplification of extracted dna?
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Develop an argument from evidence on how human activity can lead to ocean acidification? Further explain what we can do, as humans, to decrease the amount of ocean acidification occurring in aquatic environments.
Answers
Answer
Ocean acidification results from the solubility of CO2 in oceans increasing the H+ concentrations, and depletion of bicarbonates concentrations.
Therefore, human activities which increases the concentration of CO2 in the atmosphere, will raise the atmospheric CO2 concentration. CO2 is constantly being absorbed by green plants maintaining its concentration through carbon cycle,Therefore massive deforestation which removes green plants, cutting down photosynthesis is one of human activities which raises CO2 levels of the atmosphere.The CO2 dissolves in the oceans to raise its acidic levels.Lower pH.
Another source is fossil fuels emissions which add more CO2 to the atmosphere.
Explanation:
All eukaryotes are multicellular, contain organelles, and replicate via binary fission. True False
Answers
The statement is FALSE
Eukaryotes are multicellular, contain organelles, and undergo MITOSIS for cellular division.
Prokaryotes are organisms that replicate through binary fission.
What drives surface currents?
differences in air temperature
differences in climate
differences in water density
differences in water salinity
Answers
Answer:
WHAT DRIVES SURFACE CURRENTS?
Surface currents in the ocean are driven by global wind systems that are fueled by energy from the sun. Patterns of surface currents are determined by wind direction, Coriolis forces from the Earth's rotation, and the position of landforms that interact with the currents.
DIFFERENCES IN AIR TEMPERATURE.
Movement of air due to differences in temperature between two locations is known as convection or advection. Convection is the term commonly applied to vertical movement of air, whilst advection is used in the context of horizontal displacement of air.
DIFFERENCES IN CLIMATE.
Weather refers to short term atmospheric conditions while climate is the weather of a specific region averaged over a long period of time. Climate change refers to long-term changes.
DIFFERENCES IN WATER DENSITY.
High temperature makes water less dense. As water gets warmer, its molecules spread out, so it becomes less dense. As it gets colder, it becomes denser. Most chemicals get denser when they turn from a liquid to a solid, but water is different.
DIFFERENCES IN WATER SALINITY.
As we have seen, most differences in salinity are due to variations in evaporation, precipitation, runoff, and ice cover. All of these process occur at the ocean surface, not at depth, so the most pronounced differences in salinity should be found in surface waters.
Explanation:
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FILL IN THE BLANK. viruses are often isolated from cells by filtration based upon the fact that they ______.
Answers
Viruses are often isolated from cells by filtration based upon the fact that they are smaller than the pore size of the filter.
Virus, a small, basic infectious agent that can reproduce only in live cells of animals, plants, or microorganisms. The term is derived from a Latin word that means "slimy liquid" or "poison." Dmitry I, a Russian scientist, conducted the first study on the biological nature of viruses in 1892.
A virus is a submicroscopic infectious agent that multiplies solely within an organism's live cells. Viruses infect all kinds of life, including animals and plants, as well as microbes such as bacteria and archaea. They proliferate at an astonishing pace, but only in living host cells. They are acellular, which means they lack cytoplasm and cellular organelles. They have no metabolic mechanism of their own and must multiply utilising the metabolic machinery of the host cell.
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What evidence does the author provide to support the claim that biomass can help companies save money?
A Biomass can be converted to other useable forms of energy, such as methane gas, or transportation fuels, such as ethanol and biodiesel."
B About 80% of the wood and wood waste fuel used in the United States is consumed by industry, electric power producers, and commercial businesses."
C Many manufacturing plants in the wood and paper products industry use wood waste to produce their own steam and electricity."
D There are about 76 waste-to-energy plants in the United States that nenerate electricity or produce steam."
Answers
Answer:
C
Explanation:I took the test and I got 100% hope it helps
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how are temperature and energy related?
THIS IS SCIENCE
Answers
Answer:
Temperature and energy are closely related in science. Temperature is a measure of the average kinetic energy, or movement, of the particles in a substance. When the particles in an object have more kinetic energy, they move faster and collide more frequently.
As a result, the object's temperature increases. Conversely, when particles have less kinetic energy, they move slower and collide less often, causing the temperature to decrease.
Therefore, temperature can be considered as a way to measure the amount of energy present in a substance, where higher temperatures correspond to greater energy levels.
Answer:
Temperature is a measure of the average kinetic energy of the particles in a substance. Kinetic energy is the energy of motion, and it depends on the speed and mass of the particles. The faster the particles move, the more kinetic energy they have, and the higher the temperature of the substance. The relationship between temperature and energy is also affected by the specific heat capacity of the substance, which is the amount of energy needed to raise the temperature of one gram of the substance by one degree Celsius. Different substances have different specific heat capacities, depending on their molecular structure and interactions. For example, water has a high specific heat capacity, which means it takes a lot of energy to change its temperature, while metals have low specific heat capacities, which means they heat up and cool down quickly.
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Reviewing Meiosis:
1. How many cells are present and how many chromosomes are present in the cell at each stage?
start of Meiosis____
end of Meiosis 1_____
end of Meiosis 2____
Answers
1. How many cells are present and how many chromosomes are present in the cell at each stage?
start of Meiosis a parent cell that is diploid
end of Meiosis 1 when the chromosomes of each homologous pair arrive at opposing poles of the cell.
end of Meiosis 2 four haploid daughter cells
What can Inherited genetic variation be caused by?
Answers
Inherited genetic variation can be caused by simply by genetic mutation.
What is meant by Inherited genetic variation?Inherited genetic variation can simply be defined as those variants traits of characteristics which is usually, frequently and most of the time transferred from parents to offsprings. That being said, it is situation which occurs as a result of mutation.
However, traits are passed on offsprings from their parents due to the genetic makeup which comes together between the parents. Traits which can be transferred from to offsprings include the following:
Colour of the eyeHeightShape of noseIn addition to the information above, variation are those differences which exists between organisms of the same species.
In conclusion, it can be deduced from the explanation given above that germline variants are being passed from parents through mutation.
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The FBI uses STR (short tandem repeat) analysis to identify criminals, and the FBI stores the information in a database. How many regions, or loci, of DNA are used for STR analysis? 5 13 26 30
Answers
Answer:
13
Explanation:
Short tandem repeats (STRs), also known as microsatellites, are short DNA sequences containing core repeat units of between 2-7 nucleotides (nts) which are tandemly repeated. The STR analysis is commonly used in molecular biology laboratories in order to identify DNA samples by comparing STR loci between samples. For example, the Federal Bureau of Investigation (FBI) uses 13 STR loci for the identification of individuals in the USA. It is important to note that these 13 loci are independently assorted (i.e. they are not linked), and thereby their combined use results in a very low ("nearly zero") false-positive identification rate.
Answer:
bbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbb
Explanation:
just Because it is
It is crucial to study ocean microbes because _______. A. They control the major biogeochemical cycles that keep Earth’s biosphere in balance b. They are a significant source of greenhouse gases and pollution c. They are frequently used as food sources in developing nations d. They provide a significant alternative energy source used to replace coal and oil Please select the best answer from the choices provided A B C D.
Answers
The ocean or the marine microorganisms are the microbes of the sea and ocean environment. They are important because they regulate the biogeochemical cycles.
What is the importance of marine or oceanic microbes?Marine microbes are crucial and play a major role in the regulation and control of the biogeochemical cycles of the marine ecosystem. They include bacteria, fungi, archaea etc.
They regulate and influences the marine environment and are a part of the marine ecosystem and cycles. They take part in the cycles of nitrogen and carbon that includes the breaking and utilizing of certain dead materials and compounds.
Therefore, marine microbes are crucial as option A. they control the biogeochemical cycles.
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Which location on the topographic map will be
most affected by weathering?
Location W
Location X
Location Y
Location Z
Answers
The location on the topographic map affected most by weathering is A, Location W.
Why is a topography affected by weathering?Weathering can affect various parts of topography such as rock formations, soil profiles, and landforms. For example, physical weathering caused by temperature changes, wind erosion, and water erosion can break down rock formations and reshape landforms such as mountains, canyons, and valleys.
Chemical weathering caused by acid rain and other chemical reactions can alter the composition of rock and soil, affecting their properties and structure. Overall, weathering plays a significant role in shaping the topography of an area over long periods of time.
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What are the links between the environment and Covid-19? Choose all correct answers.
A. with the destruction of ecosystems the natural barriers between host animals is reduced
B. We destroy ecosytems creating channels for viruses to pass to humans
C. a decrease in human population is decreasing resource use including wild animals
D. destruction of biodiversity
E. an increase in forest ecosystems
F. deforestation
Answers
The correct answers that describe the links between the environment and Covid-19 are options A. With the destruction of ecosystems, the natural barriers between host animals are reduced, B. We destroy ecosystems, creating channels for viruses to pass to humans, D. Destruction of biodiversity, F. Deforestation.
With the destruction of ecosystems, the natural barriers between host animals are reduced: The destruction of ecosystems, such as deforestation or habitat loss, can lead to increased contact between humans and wildlife. This close proximity increases the chances of zoonotic diseases, like Covid-19, transferring from animals to humans.
We destroy ecosystems, creating channels for viruses to pass to humans: Human activities that involve destroying natural habitats, encroaching upon wildlife, or engaging in the illegal wildlife trade create opportunities for viruses to jump species barriers and infect humans. Encroachment into natural areas can disrupt the balance of ecosystems, bringing humans in contact with new pathogens.
Destruction of biodiversity: Biodiversity plays a crucial role in maintaining healthy ecosystems and acting as a buffer against disease outbreaks. When biodiversity is reduced, ecosystems become more vulnerable, and the spread of diseases can be intensified.
Deforestation: Deforestation, the clearing of forests at a large scale, can disrupt ecosystems, displace wildlife, and increase the likelihood of contact between humans and potential disease-carrying animals. It can also lead to changes in local climate patterns, which may indirectly influence the spread of diseases.
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which of the following describes a reason for making a prediction when doing a controlled experiment
a. To understand the nature of an experimental subject
b. To provide a context for why a specific question was asked
c. To indicate what specific results will support a hypothesis
d. To identify a useful and reliable source of scientific information
Answers
To indicate what specific results will support a hypothesis.
Experiment plays many roles in science. One of its important roles is to test theories and to provide the basis for scientific knowledge.What is hypothesis?A hypothesis is an assumption, an idea that is proposed for the sake of argument so that it can be tested to see if it might be true.In the scientific method, the hypothesis is constructed before any applicable research has been done, apart from a basic background review.To know more about experiment here
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what is the advantage of using the standard plate count over other enumeration methods when determining the safety of a food or water sample? multiple choice it provides a count of only living bacteria which represent the safety concern. it allows for a count of bacteria that are not easy to culture. it takes very little time and materials. it counts all bacteria, living and dead, to provide an overall picture of organism density.
Answers
The standard plate count approach is better for assessing food and water safety since it counts only live bacteria. The classic plate count method includes inoculating a diluted sample onto an agar medium and letting the bacteria develop into visible colonies.
Each colony is a live, potentially harmful bacterium. Counting colonies estimates the sample's live bacteria. For sample safety, this approach counts live bacteria. Living bacteria can grow and reproduce, making them most likely to cause foodborne or waterborne infections. The standard plate count approach better estimates sample risk by counting only live bacteria.
Microscopic examination or DNA-based approaches may count living and dead bacteria or non-viable cells. These approaches may overestimate bacterial density and safety by not distinguishing viable from non-viable bacteria.
Thus, the standard plate count method targets living bacteria, making it easier to assess food or water sample safety.
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The term used to describe a location with many different cultural groups is
A. culturally diverse
B. globalization
C. multiple perspectives
D. ethnicity
Answers
It’s the only answer that even makes sense if you really think about it.
How do different parts of the digestive system help to break down fat molecules so they can be absorbed into the body.
Answers
Different parts of the digestive system, including the mouth, stomach, liver, gallbladder, and small intestine, work together to break down fat molecules for absorption into the body.
The digestive system plays a crucial role in breaking down fats into smaller molecules that can be absorbed by the body. The process starts in the mouth, where chewing and the action of saliva begin to break down fats mechanically. However, the main breakdown of fats occurs in the small intestine.
When fats reach the small intestine, bile produced by the liver and stored in the gallbladder is released. Bile helps to emulsify fat, breaking it down into smaller droplets, which increases the surface area for enzymes to act upon. Pancreatic enzymes, specifically lipase, are then released into the small intestine, where they break down fats into fatty acids and glycerol. These smaller molecules can be easily absorbed by the intestinal lining and transported into the bloodstream.
The breakdown of fats in the digestive system is facilitated by a combination of mechanical and enzymatic processes, involving various organs and enzymes. This ensures efficient digestion and absorption of fats, providing the body with essential nutrients for energy production and other physiological functions.
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All of the following are characteristic of replication of the E. coli circular chromosome EXCEPT: replication is bi-directional. the unwinding of the duplex DNA is driven by the translocation of the DNA polymerase torsional stress introduced in the duplex DNA is relieved by DNA gyrase replication forks move in opposite directions
Answers
The characteristic of replication of the E. coli circular chromosome that is NOT true is "replication forks move in opposite directions." In E. coli, replication is bi-directional, meaning that two replication forks move in opposite directions from a single origin of replication.
The unwinding of the duplex DNA is driven by the translocation of the DNA polymerase, and torsional stress introduced in the duplex DNA is relieved by DNA gyrase. These processes ensure that the replication of the chromosome is accurate and complete. Once the replication forks have moved around the entire circular chromosome, the replication process is complete. Overall, E. coli replication is a complex process that involves the coordination of multiple enzymes and proteins to ensure that the genetic information is accurately copied and passed on to the next generation of cells.
Hi there! The statement that is NOT characteristic of the replication of the E. coli circular chromosome is: "the unwinding of the duplex DNA is driven by the translocation of the DNA polymerase." In reality, the unwinding of duplex DNA is carried out by helicases, not DNA polymerase. The other statements accurately describe E. coli replication. Replication is bi-directional, meaning it proceeds in both directions from the origin. DNA gyrase relieves the torsional stress introduced in the duplex DNA during replication. Additionally, replication forks, which are the points at which the DNA is unwound and replicated, do move in opposite directions as the replication process continues.
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significance of photosynthesis
Answers
Answer:
Significance of Photosynthesis. Photosynthesis is the process by which green plants, in the presence of light combine water and carbon dioxide to form carbohydrates. Oxygen is released as a by product of photosynthesis.
Explanation:
which of the following statements is true? a.) pyruvate is produced when a glucose molecule undergoes glycolysis. b.) pyruvate is a byproduct of atp production. c.) glycolysis turns pyruvate into glucose. d.) phosphorylation is another word for glycolysis.
Answers
When a glucose molecule engages in glycolysis, pyruvate is formed.
Explain glycolysis:Nearly all sorts of organisms have evolved a metabolic process and anaerobic energy source called glycolysis. The Embden-Meyerhof pathway is another name for the process, given in honour of the key figures in its discovery and comprehension. It serves a purpose in anaerobic respiration because it doesn't need oxygen, but it is also the initial stage in cellular respiration. Molecules of glucose, the most important organic fuel for plants, microorganisms, and animals, are oxidized during the process. A majority of cells favor glucose (although there are exceptions, such as acetic acid bacteria that prefer ethanol). Per glucose molecule, glycolysis uses up 2 ATP molecules and generates 4 ATP, 2 NADH, and 2 pyruvates.
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the null hypothesis and alternative hypothesis, when combined, cover the entire sample space of the parameter of interest. true or false.
Answers
The given statement is False, the null hypothesis and alternative hypothesis do not collectively cover the entire sample space of the parameter of interest.
Do the null hypothesis and alternative hypothesis cover the entire sample space of the parameter of interest?The null hypothesis and alternative hypothesis do not collectively cover the entire sample space of the parameter of interest.
In hypothesis testing, the null hypothesis (H0) represents the default or no effect assumption, while the alternative hypothesis (Ha) states the specific effect or relationship that the researcher wants to investigate.
These hypotheses are mutually exclusive and collectively exhaust the possibilities for the parameter of interest.
However, it is important to note that the null hypothesis does not cover the entire sample space, as it only represents the absence of an effect or difference.
The alternative hypothesis, on the other hand, specifies a particular effect or difference that is being tested against the null hypothesis.
Thus, the two hypotheses together cover all the possible outcomes but not the entire sample space of the parameter being studied.
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Which trait is not a genetic trait?
O A. Freckled skin
B. Long hair
O C. Detached earlobes
O D. Hazel eyes
Answers
Answer:
long hair
Explanation:
the __________ is the largest and most prominent of the laryngeal cartilages.
Answers
The thyroid cartilage is the largest and most prominent of the laryngeal cartilages. The larynx, also known as the "Adam's apple," is built from a single shield-shaped cartilage called the thyroid cartilage.
Due to differences in the hormonal impacts during puberty, it is more evident in men than in women and is positioned in the anterior (front) region of the neck.
Two laminae (plates), which make up the thyroid cartilage, come together in the middle to produce the laryngeal prominence, often known as the Adam's apple. It offers defense to the laryngeal structures, especially the vocal folds.
Individual differences exist in the size and prominence of the thyroid cartilage, which is typically greater in adult males. By enclosing the vocal cords, in addition to playing a protective role, it also aids in sound generation and vocalization.
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Which hormone is only used by males?
O A. Progesterone
B. Testosterone
C. Luteinizing hormone
D. Follicle-stimulating hormone
Answers
Answer:
Testosterone is the primary male sex hormone
Explanation:
Progesterone occurs in the body naturally or made in the lab; relearsed by the ovaries to aid in menstrual/menopausal symtpoms or for the implantation of fertized eggs for pregnancy.
Luteinizing hormone helps your reproductive system: specifically, a woman’s ovaries and a man’s testes; used to test for infertility or check if the pituitary gland have issues.
Follicle-stimulating hormone is essential during puberty development in males and females (ovaries and testes)
The hormone used only in males is testosterone. Therefore, the correct option is B.
Testosterone is a hormone that is primarily associated with male reproductive development and function.
It plays a key role in the development of male secondary sexual characteristics, such as facial and body hair growth, deepening of the voice, and muscle mass development.
Testosterone is produced primarily in the testes, although small amounts are also produced in the adrenal glands of both males and females.
In males, testosterone is responsible for regulating sperm production and maintaining overall reproductive health.
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A cell can use this type of bulk transport to bring something large inside the cell
Answers
Answer:
Phagocytosis.
Explain what sustainable Forest Management is
Answers
Answer:
Basically helping, and taking care of the forest. Without destroying anything a future gen. might feel is the same
Explanation:
The practice of meeting the forest resource needs and values of the present without compromising the similar capability of future generations
what are the products in cellular respiration
Answers
Answer:
In cellular respiration, glucose and oxygen react to form ATP. Water and carbon dioxide are released as byproducts. The three stages of aerobic cellular respiration are glycolysis (an anaerobic process), the Krebs cycle, and oxidative phosphorylation.